|
KMID : 0376219730100010181
|
|
Chonnam Medical Journal
1973 Volume.10 No. 1 p.181 ~ p.189
|
|
|
Effect of Adenosine Diphosphate Ribose on the Glycolysis of Rabbit Brain Homogenate
|
|
|
|
|
Abstract
|
|
|
The effects of adenosine diphosphate ribose(ADP-ribose), a product from NAD by the action of -NAD glycobydrolase, on the glycolytic capacity of rabbit brain homogenate were studied in a system fortified -with ATP, NAD, fructose-l,6- diphosphate, nicotinamide, and MgCl2. The glycolytic capacityy was assessed by the measurement of aerobic glucose consumption and anaerobic lactate production.
The fortified brain homogenate showed a high glycolytic capacity, and omission of anyone of the cofactors caused a decrease in the glycolysis. ATP could be replaced with AMP. AMP added in place of ATP or added together with ATP caused a slight increase in the glucose consumption.
ADP-ribose in a concentration of 0.5 to 1.0 mM caused a decrease in the glycolysis at 60 minutes of incubation. In contrast, ADP-ribose in a low conceeatration(less than 0.2mM), and within 20 minutes of incubation, caused a increase in.the glycolysis.
Ribose-5´-phosphate, which will be produced enzymically from ADP-ribose, also caused a decrease in the glycolysis in a concentration range of ADP-ribose used.
The enzyme, ADP-ribose pyrophosphohydrolase, which hydrolyzes ADP-ribose to produce ribose-5´-phosphate and AMP, was detected along with high activity of NAD glycohydrolase in rabbit brain.
From these results, it is suggested that the inhibitory -effect of ADP-ribose in high concentrations on the glycolysis is due to ribose-5´-phosphate which is produced from ADP-ribose by ADP-ribose pyrophosphchydrolase. It seems also likely that the enhancing effect on the glycolysis of ADP-ribose in low concentrations may, be due to AMP, which is produced from ADP-ribose enzymatically and activates phosphofructokinase of the brain.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|